Tertiary butyl hydroperoxide induced oxidative damage in mice erythrocytes: Protection by taurine
- 作者：Anandita Roy ; Parames C. Sil ; parames@bosemain.boseinst.ac.in ; parames_95@yahoo.co.in
- 关键词：TBHP ; tertiary butyl hydroperoxide ; ROS ; reactive oxygen species ; GSH ; glutathione ; GSSG ; glutathione disulfide ; CAT ; catalase ; GR ; glutathione reductase ; GST ; glutathione-S-transferase ; SOD ; superoxide dismutase ; GPx ; glutathione peroxidase ; G-6-PD ; gluc
- 刊名：Pathophysiology
- 出版年：2012
- 期刊代码：254_09284680
- 类别：med
- 出版时间：April, 2012
- 卷：19
- 期：2
- 页码：137-148
- 文件大小：1084 K
摘要
The present study was undertaken to investigate the protective role of taurine, against t-butyl hydroperoxide (TBHP) induced oxidative stress in murine erythrocytes. Erythrocytes were treated either with TBHP alone or with taurine, followed by TBHP exposure. TBHP-induced oxidative stress increased methemoglobin formation, lipid peroxidation and protein carbonylation in erythrocytes. The same exposure, however, depleted cellular GSH content and altered the activities of the antioxidant enzymes as well as of methemoglobin reductase; reduced activities of Ca+ and Na+/K+ ATPase and intracellular ATP levels. Taurine transport inhibitor, 尾-alanine, treated erythrocytes showed increased phosphatidylserine externalization and ROS formation on TBHP exposure and taurine could not revert the effect. TBHP exposure increased intracellular calcium and upregulated the level of calpain. Administration of taurine could, however, prevent the TBHP induced oxidative imbalance. Electron micrographs of erythrocytes showed changed morphology with an increase in the number of echinocytes. Taurine treatment could restore the normal levels of the antioxidant enzymes and metabolites of the erythrocytes. Results suggest that the oxidative insult introduced in erythrocytes by TBHP administration is prevented by taurine mainly via membrane stabilization.