Traditional extract of Pithecellobium dulce fruits protects mice against CCl₄ induced renal oxidative impairments and necrotic cell death

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• 作者：Pabitra Bikash Pal ; Sankhadeep Pal ; Prasenjit Manna ; Parames C. Sil ; ^{parames@bosemain.boseinst.ac.in} ; ^{parames_95@yahoo.co.in}
• 关键词：AEPD ; aqueous extract of the fruits of Pithecellobium dulce ; BSA ; bovine serum albumin ; BUN ; blood urea nitrogen ; CCl4 ; carbon tetrachloride ; CDNB ; 1-chloro-2 ; 4-dinitrobenzene ; DPPH ; 2 ; 2-diphenyl-1-picrylhydrazyl ; DTNB ; 5 ; 5&prime ; -dithiobis(2-nitrobenzoic
• 刊名：Pathophysiology
• 出版年：2012
• 期刊代码：254_09284680
• 类别：med
• 出版时间：April, 2012
• 卷：19
• 期：2
• 页码：101-114
• 文件大小：1040 K

摘要

The present study has been carried out to investigate the role of the aqueous extract of the fruits of Pithecellobium dulce (AEPD) against carbon tetrachloride (CCl₄) induced renal oxidative injury in mice. HPLC analysis shows that AEPD contains phenolics, flavonoids and saponins as the major active components. Creatinine and blood urea nitrogen (BUN) levels were assayed to determine renal protective action of AEPD in CCl₄-induced renal pathophysiology. Its antioxidant activity was determined by measuring radical scavenging activity, antioxidant enzymes activities, GSH content, protein carbonylation and lipid peroxidation. In addition, FACS analysis, DNA fragmentation and histological studies were carried out to determine its effect in CCl₄ induced renal oxidative injury and cell death. CCl₄ exposure increased the intracellular reactive oxygen species production, decreased intracellular antioxidant defence, reduced mitochondrial membrane potential, attenuated the intracellular ATP content and caused renal cell death mainly via the necrotic pathway as revealed by DNA fragmentation analysis. Treatment with AEPD both prior and post to the toxin exposure protected the organ from CCl₄ induced oxidative insult. Histological studies also support our results. Combining, results suggest that the protective role of AEPD against CCl₄ induced renal oxidative impairments is probably due to the antioxidative properties present in its active constituents.