The thiol enzyme from rat spleen that produces bradykinin potentiating peptide from rat plasma

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• 作者：Yamafuji ; Keiko ; Taniguchi ; Yoriko ; Sakamoto ; Eri
• 关键词：Thiol protease ; Bradykinin potentiating peptide ; Proline rich peptide
• 刊名：Immunopharmacology
• 出版年：1996
• 期刊代码：43_01623109
• 类别：imm
• 出版时间：May, 1996
• 卷：32
• 期：1-3
• 页码：157-159
• 文件大小：206 K

摘要

We have focused our studies on a thiol-dependent enzyme of 37 kilodaltons (kDa) that produces a bradykinin (BK) potentiating peptide. The molecular mass of the peptide was estimated to be around 750 Da and its amino acid composition was Pro₄Gly₂ Leu₁ Ser₁ with a proline and a serine at the N- and C-terminals, respectively. Biological activity was assayed by means of uterus contraction. The enzyme differs from Cathepsin L or B by virtue of its immunological reactivity and enzyme kinetics. The inhibitors used were leupeptin, antipain, E-64 and chymostatin in order of effectiveness. The low molecular SH reagents also diminished the enzyme activity. Among the metal ions tested, Cu²⁺ and Zn²⁺ inhibited the reaction.