Flexible interaction of Drosophila Smad complexes with bipartite binding sites
- 作者：Sheng Gao ; Allen Laughon
- 关键词：Smad ; Mad ; Medea ; Dpp ; Brinker ; BMP
- 刊名：Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression
- 出版年：2007
- 期刊代码：15_01674781
- 类别：bio
- 出版时间：July-August 2007
- 卷：1769
- 期：7-8
- 页码：484-496
- 文件大小：1604 K
摘要
A subset of BMP-responsive enhancer elements are characterized by pairing of a GC-rich Smad1 binding site and an SBE-type Smad4 binding site. Such paired, or bipartite, sites are in some cases just 5 bp apart and thus might be contacted by a single Smad1–Smad4 complex. Other potential pairings are separated as much as 60 bp but it is not known whether such longer distances can be spanned by a Smad1–Smad4 complex, indeed binding of native Smad1–Smad4 complexes to any of these bipartite elements has yet to be reported. Here we report that a complex of the homologous Drosophila Smad proteins, Mad and Medea, is capable of concerted binding to GC-rich and SBE sites separated by as much as 20 bp. The wider the separation, the more severely binding affinity was reduced by shortening of the linker region that tethers the DNA binding domain of Medea. In contrast, length of the Mad linker did not affect the allowed distance between paired sites, rather it contributes specifically to Mad contact with the GC-rich site. Finally, we show that Smad1 and Smad4 can participate in binding to bipartite sites.