Reduction of bromate in a biological activated carbon filter under high bulk dissolved oxygen conditions and characterization of bromate-reducing isolates

详细信息	查看全文 | 推荐本文 |

• 作者：Juan Liu ; Jianwei Yu ; Dong Li ; Yu Zhang ; Min Yang ; ^{yangmin@rcees.ac.cn}
• 关键词：Biofilters ; Bromate-reducing bacteria ; Dissolved oxygen ; Biofilms ; Biotransformation ; Characterization
• 刊名：Biochemical Engineering Journal
• 出版年：2012
• 期刊代码：9_1369703x
• 类别：ce
• 出版时间：15 June, 2012
• 卷：65
• 期：Complete
• 页码：44-50
• 文件大小：489 K

摘要

A biological activated carbon (BAC) filter was constructed using BAC from a pilot system that exhibited the ability to reduce bromate (BrO₃^{鈭?/sup>) and two BrO₃鈭?/sup>-reducing bacteria were isolated and characterized. The BAC filter could almost completely reduce BrO₃鈭?/sup> (60 渭gBr/L) to bromide (Br鈭?/sup>) at an influent dissolved oxygen (DO) level of approximately 8.0 mg/L and an empty bed contact time of 30 卤 2 min using acetate as the electron donor shortly after the start-up. Phylogenetic analysis of the 16S rRNA gene sequences of a biological sample from the BAC filter showed that among the six detected orders, Rhodocyclales- and Burkholderiales-related microorganisms were dominant and Rhodocyclaceae- and Comamonadaceae-related microorganisms may play a role in BrO₃鈭?/sup> reduction. Two isolated pure cultures, i.e. Sphingomonas sp. 4721 and Deinococcus sp. 4710, exhibited the ability to reduce BrO₃鈭?/sup> in the presence of NO₃鈭?/sup>. The result of this study clearly indicated that DO was a competitor of BrO₃鈭?/sup> as an electron acceptor while NO₃鈭?/sup> was not. Construction of a BAC filter which could restrict oxygen transfer within a biofilm still remains to be a challenge.}