Purification and characterisation of a novel cysteine conjugate β-lyase from the tapeworm Moniezia expansa

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• 作者：Adcock ; Harriet J. ; Brophy ; Peter M. ; Teesdale-Spittle ; Paul H. ; Buckberry ; Lorraine D.
• 关键词：Cysteine conjugate β ; -lyase ; Moniezia expansa ; Parasite
• 刊名：International Journal for Parasitology
• 出版年：2000
• 期刊代码：124_00207519
• 类别：med
• 出版时间：April 15, 2000
• 卷：30
• 期：5
• 页码：567-571
• 文件大小：112 K

摘要

The paper presents the first report of the purification of an invertebrate cysteine conjugate β-lyase (CCBL). CCBL activity was shown to predominate within the cytosolic fraction of tissue from the tapeworm Moniezia expansa. The monomeric cytosolic enzyme was isolated with a M_r of 72 kDa and co-purified with transaminase activity towards l-aspartate. The substrate profile for M. expansa CCBL is different from that of mammalian CCBLs. Exploiting the differences in mammalian and parasite substrate profiles will facilitate the development of helminth targeted conjugates which will not be activated by host (mammalian) CCBLs.