摘要
m>Brucellam> is gram-negative bacteria responsible for brucellosis in a wide variety of animals and humans. BALB/c mice were immunized with live m>Escherichia colim> expression the m>p39m> gene of m>Brucella melitensism>, a gene coding for the periplasmic binding protein. Mice were injected with either m>E.聽colim> BL21 (DE3) pEt15b or m>E.聽colim> BL21 (DE3) pEt15b-p39 alone or adjuvanted with either CpG oligodeoxynucleotides (CpG ODN) or non-CpG ODN. m>E.聽colim> BL21 (DE3) pEt15b-p39 with CpG ODN or with non-CpG ODN mice groups showed a significant IFN-纬 production and T-cell proliferation as a reaction to P39 antigen. In addition, antibody responses (IgG, IgG1 and IgG2a), were only found in these two mice groups. A higher level of protection against m>B.聽melitensism> 16M were observed in mice immunized with m>E.聽colim> BL21 (DE3) pEt15b-p39 and CpG ODN comparing with those immunized with m>E.聽colim> BL21 (DE3) pEt15b-p39 alone or with non-CpG ODN. No protection against m>B.聽melitensism> 16M was observed in mice immunized with m>E.聽colim> BL21 (DE3) pEt15b alone or with the adjuvant. Rev.1 protection at 4 and 8 weeks post-challenge was more effective than that observed with m>E.聽colim> BL21 (DE3) pEt15b-p39 and CpG ODN.
