Construction of new stable strain over-expressing the glucose isomerase of the Streptomyces sp. SK strain

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• 作者：Monia Mezghani ; Mohamed Ali Borgi ; Radhouane Kammoun ; Hedi Aouissaoui and Samir Bejar
• 关键词：Glucose isomerase ; Over-expression ; CBS4 strain ; ermE-up promoter
• 刊名：Enzyme and Microbial Technology
• 出版年：2005
• 期刊代码：26_01410229
• 类别：ce
• 出版时间：2005
• 卷：37
• 期：7
• 页码：735-738
• 文件大小：169 K

摘要

In order to over express the xylA gene of Streptomyces sp. SK strain, it was cloned under the control of the constitutive ermE-up promoter. This construct was integrated through site-specific recombination process into the chromosome of a Streptomyces violaceoniger glucose isomerase deficient strain using the non-replicative vector pTS55. The resulting CBS4 strain shows a perfect stability in the absence of selection pressure. Its glucose isomerase activity was about four and nine-fold greater, than that obtained from Streptomyces sp. SK, respectively fully induced or not by xylose.