Bioinformatical evaluation of modified nucleosides as biomedical markers in diagnosis of breast cancer
- 作者：Dino Bullinger ; Holger Frö ; hlich ; Fabian Klaus ; Hans Neubauer ; Antje Frickenschmidt ; Carsten Henneges ; Andreas Zell ; Stefan Laufer ; Christoph H. Gleiter ; Hartmut Liebich ; Bernd Kammerer
- 关键词：Metabolomics ; High performance liquid chromatography with ultraviolett detection (HPLC-UV) ; Nucleosides ; Breast cancer ; Support Vector Machine (SVM) ; k-Nearest-neighbor classifier (k-NN)
- 刊名：Analytica Chimica Acta
- 出版年：2008
- 期刊代码：2_00032670
- 类别：ch
- 出版时间：16 June 2008
- 卷：618
- 期：1
- 页码：29-34
- 文件大小：381 K
摘要
It is known that patients suffering from cancer diseases excrete increased amounts of modified nucleosides with their urine. Especially methylated nucleosides have been proposed to be potential tumor markers for early diagnosis of cancer. For determination of nucleosides in randomly collected urine samples, the nucleosides were extracted using affinity chromatography and then analyzed via reversed phase high-performance liquid chromatography (HPLC) with UV-detection. Eleven nucleosides were quantified in urine samples from 51 breast cancer patients and 65 healthy women.