Structure, fluorescence quenching and antioxidant activity of a carbohydrate polymer from Eugenia jambolana

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• 作者：Shruti S. Bandyopadhyay^a ; ¹ ; Debjani Ghosh^a ; ¹ ; Valé ; rie Micard^b ; Sharmistha Sinha^a ; Udipta R. Chatterjee^a ; Bimalendu Ray^a ; ^{bimalendu_ray@yahoo.co.uk}
• 关键词：BHA ; butylated hydroxy anisole ; BHT ; butylated hydroxy toluene ; BSA ; bovine serum albumin ; DPPH ; 1 ; 1-diphenyl-2-picrylhydrazyl ; FRAP ; ferric reducing antioxidant potential ; FT-IR ; Fourier transform infra red ; GLC/MS ; gas liquid chromatography/mass spectro
• 刊名：International Journal of Biological Macromolecules
• 出版年：2012
• 期刊代码：62_01418130
• 类别：abs
• 出版时间：July-August, 2012
• 卷：51
• 期：1-2
• 页码：158-164
• 文件大小：568 K

摘要

Natural products provide an excellent source for novel antioxidants. Herein, we have studied the water-extracted carbohydrate polymer (WE) of Eugenia jambolana using chemical, chromatographic, and spectroscopic methods. A 116 kDa arabinogalactan containing p-coumaric and ferulic acids in monomeric and dimeric forms has been isolated. Cellulase generated oligomeric fragments containing ester linked phenolic acids were also characterized. The antioxidant capacity of this carbohydrate polymer is comparable to butylated hydroxy anisole and butylated hydroxy toluene. Interaction of WE with bovine serum albumin (BSA) was studied by fluorescence quenching measurement. Conformational change of BSA at high carbohydrate polymer concentration was indicated.