The Caco2 cell cytoskeleton was disrupted with either cytochalasin D, phalloidin, colchicine, or paclitaxel. Levels of α-actinin-1 and -4 and paxillin were reduced by specific small interfering RNA. Cells on collagen I–precoated membranes were subjected to 10%repetitive deformation at 10 cycles/min. After 1 hour, cells were lysed for Western blot analysis.
Strain-activated ERK1/2, focal adhesion kinase, and Src phosphorylation in dimethyl sulfoxide- and/or nontargeting small interfering RNA-treated control cell populations. Cytochalasin D and paclitaxel, but not phalloidin and colchicine, blocked ERK1/2 phosphorylation. A decrease in α-actinin-1, but not in α-actinin-4 or paxillin, inhibited ERK1/2 and focal adhesion kinase phosphorylation, whereas Src activation appears to be independent of these effects.
The intestinal epithelial cell cytoskeleton may transduce mechanical signals by way of α-actinin-1 into the focal adhesion complex, culminating in ERK1/2 activation and proliferation.