Differential subcellular localization of DNA topoisomerase-1 isoforms and their roles during Caenorhabditis elegans development

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• 作者：Dong Seok Cha^a ; ¹ ; Sarah E. Hollis^a ; Udaya Sree Datla^c ; Sejin Lee^d ; Jinsun Ryu^d ; Hey Rim Jung^e ; Eunsuk Kim^a ; Kyuhyung Kim^f ; Myeongwoo Lee^g ; Chris Li^h ; Myon-Hee Lee^a ; ^b ; ^c ; ⁱ ; ^{leemy@ecu.edu}
• 关键词：Caenorhabditis elegans ; Topoisomerase-1 ; Neuron ; Chromosomal segregation ; Germline development
• 刊名：Gene Expression Patterns
• 出版年：2012
• 期刊代码：26_1567133x
• 类别：neu
• 出版时间：May-June, 2012
• 卷：12
• 期：5-6
• 页码：189-195
• 文件大小：683 K

摘要

DNA topoisomerase-1 (TOP-1) resolves the topological problems associated with DNA replication, transcription and recombination by introducing temporary single-strand breaks in the DNA. Caenorhabditis elegans TOP-1 has two isoforms, TOP-1伪 and TOP-1尾. TOP-1尾 is broadly localized to the nuclei of many cells at all developmental stages and concentrated in nucleoli in embryo gut and oogenic cells. However, TOP-1伪 is specifically localized to centrosomes, neuronal cells, excretory cells and chromosomes of germ cells in embryonic and larval stages. Reporter gene analysis also shows that top-1 transcription is highly activated in several sensory neurons, speculating the possible role of TOP-1伪 in neuronal development. From RNA interference (RNAi) experiments, we demonstrated that C. elegans TOP-1 is required for chromosomal segregation, germline proliferation and gonadal migration, which are all correlated with the expression and activity of TOP-1. Therefore, our findings may provide an insight into a new role of TOP-1 in development of multicellular organisms.