Chromium exhibits adverse effects at environmental relevant concentrations in chronic toxicity assay system of nematode Caenorhabditis elegans
- 作者：Quili Wu ; Yangyang Qu ; Xing Li ; Dayong Wang ; dayongw@seu.edu.cn
- 关键词：Chromium exposure ; Chronic toxicity ; Environmental relevant concentration ; Caenorhabditis elegans
- 刊名：Chemosphere
- 出版年：2012
- 期刊代码：15_00456535
- 类别：env
- 出版时间：June, 2012
- 卷：87
- 期：11
- 页码：1281-1287
- 文件大小：551 K
摘要
Here we investigated whether the assay system (10-d) in Caenorhabditis elegans can be used to evaluate chronic toxicity of chromium (Cr(VI)) at environmental relevant concentrations ranging from 5.2 渭g L鈭? to 260 渭g L鈭?. The results indicated that lethality, locomotion behavior as revealed by head thrash, body bend, and forward turn, metabolism as revealed by pumping rate and mean defecation cycle length, intestinal autofluorescence, and ROS production were severely altered in Cr chronically exposed nematodes at environmental relevant concentrations. The most surprising observations were that head thrash, body bend, intestinal autofluorescence, and ROS production in 13 渭g L鈭? Cr exposed nematodes were significantly influenced. The observed adverse effects of Cr on survival, locomotion behavior, and metabolism were largely due to forming severe intestinal autofluorescence and ROS production. Therefore, our findings demonstrate the usefulness of chronic toxicity assay system in C. elegans in evaluating the chronic toxicity of toxicants at environmental relevant concentrations.