Activation of the Caenorhabditis elegans FOXO family transcription factor DAF-16 by pathogenic Bacillus thuringiensis

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• 作者：Jun Wang^a ; Rania Nakad^b ; Hinrich Schulenburg^a ; ^b ; ^{hschulenburg@zoologie.uni-kiel.de}
• 关键词：Caenorhabditis elegans ; Bacillus thuringiensis ; FOXO transcription factor ; Insulin signalling ; Innate immunity
• 刊名：Developmental and Comparative Immunology
• 出版年：2012
• 期刊代码：35_0145305x
• 类别：abs
• 出版时间：May, 2012
• 卷：37
• 期：1
• 页码：193-201
• 文件大小：909 K

摘要

The FOXO family of transcription factors have recently been implicated in innate immunity, especially in case of DAF-16 from the nematode Caenorhabditis elegans. However, previous studies with this nematode proposed that DAF-16 is not directly activated by pathogens. Rather, DAF-16 mediates resistance if activated by some other cue as part of a general stress response. We specifically tested this notion by analysis of DAF-16 nuclear translocation and thus regulatory activity upon exposure to pathogenic Bacillus thuringiensis. Our results demonstrate that DAF-16 nuclear translocation is indeed particularly induced in response to bacterial pathogenicity, whereas infection load alone has little effect. Translocation is strongest at an early time point, suggesting a role during the immediate immune response. The increased DAF-16 availability is associated with higher resistance and a reduction in feeding behaviour. Taken together, our data highlight that a FOXO transcription factor directly responds to pathogens and thus contributes to immune defence.