Multiple roles of the furrow deepening Ca²⁺ transient during cytokinesis in zebrafish embryos

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• 作者：Wai Ming Li ; Sarah E. Webb ; Ching Man Chan ; Andrew L. Miller
• 关键词：Cytokinesis ; Ca²⁺ ; IP₃ receptors ; Contractile band ; Pericleavage F-actin enrichments ; Calpains ; VAMP-2 ; Microtubules ; Kif23 ; Zebrafish embryos
• 刊名：Developmental Biology
• 出版年：2008
• 期刊代码：167_00121606
• 类别：bio
• 出版时间：15 April 2008
• 卷：316
• 期：2
• 页码：228-248
• 文件大小：4720 K

摘要

The generation of a required series of localized Ca²⁺ transients during cytokinesis in zebrafish embryos suggests that Ca²⁺ plays a necessary role in regulating this process. Here, we report that cortical actin remodeling, characterized by the reorganization of the contractile band and the formation during furrow deepening of pericleavage F-actin enrichments (PAEs), requires a localized increase in intracellular Ca²⁺, which is released from IP₃-sensitive stores. We demonstrate that VAMP-2 vesicle fusion at the deepening furrow also requires Ca²⁺ released via IP₃ receptors, as well as the presence of PAEs and the action of calpains. Finally, by expressing a dominant-negative form of the kinesin-like protein, kif23, we demonstrate that its recruitment to the furrow region is required for VAMP-2 vesicle transport; and via FRAP analysis, that kif23 localization is also Ca²⁺-dependent. Collectively, our data demonstrate that a localized increase in intracellular Ca²⁺ is involved in regulating several key events during furrow deepening and subsequent apposition.