Aeromonas hydrophila induced head kidney macrophage apoptosis in Clarias batrachus involves the activation of calpain and is caspase-3 mediated

详细信息	查看全文 | 推荐本文 |

• 作者：Chaitali Banerjee^a ; Ramansu Goswami^a ; Gaurav Verma^b ; Malabika Datta^b ; Shibnath Mazumder^a ; ^{shibnath1@yahoo.co.in}
• 关键词：Aeromonas hydrophila ; Head kidney macrophage ; Apoptosis ; Calcium ; Calpain ; Caspase-3
• 刊名：Developmental and Comparative Immunology
• 出版年：2012
• 期刊代码：35_0145305x
• 类别：abs
• 出版时间：July, 2012
• 卷：37
• 期：3-4
• 页码：323-333
• 文件大小：954 K

摘要

The mechanism of macrophage cytotoxicity induced by Aeromonas hydrophila is yet unresolved. We observed A. hydrophila induces Head Kidney Macrophage (HKM) apoptosis in Clarias batrachus, as evident from Hoechst 33342 and AnnexinV-Propidium Iodide staining and presence of oligonucleosomal DNA ladder. Initiation of apoptosis required the bacteria to be alive, be actively phagocytosed into HKM and was dependent on host proteins. Elevated cytosolic calcium and consequent calpain activity that declined following pre-incubation with EGTA, verapamil and nifedipine implicates the role of calcium influx through voltage gated calcium channels and calpain in A. hydrophila-induced HKM apoptosis. Though, calpain-1 and -2 were involved, calpain-2 appeared to be more important in the process. EGTA, verapamil, nifedipine and calpain-2 inhibitor reduced caspase-3 activity and apoptosis. We conclude that A. hydrophila alters cytosolic calcium homeostasis initiating the activation of calpains, more specifically calpain-2, which leads to caspase-3 mediated HKM apoptosis in C. batrachus.