3D molecular modeling, free radical modulating and immune cells signaling activities of the novel peptidomimetic l-glutamyl-histamine: possible immunostimulating role

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• 作者：Babizhayev ; Mark A. ; Semiletov ; Yuri A. ; Lul芒芒芒kin ; Yuri A. ; Sakina ; Natalia L. ; Savel芒芒芒yeva ; Ekaterina L. ; et. al.
• 关键词：l-Glutamyl-histamine ; l-Glutamyl-tryptophan ; Peptidomimetics ; Free radical oxygen species ; Superoxide radical ; Iron chelating and ferroxidase activities ; Immune cells signaling ; Spleen lymphocytes ; Blood mononuclear cells ; Immunostimulating activities ; Ly
• 刊名：Peptides
• 出版年：2005
• 期刊代码：59_01969781
• 类别：neu
• 出版时间：April, 2005
• 卷：26
• 期：4
• 页码：551-563
• 文件大小：291 K

摘要

An original representative of the patented by author family of histamine-containing peptidomimetics l-glutamyl-histamine (l-Glu-Hist) was synthesized and characterized as a biologically active compound with a role of cytokine mimic leading to cellular responses of improved specificity. The study assesses the ability of l-Glu-Hist to affect molecular modeling, modulate free radical activity and influence immune cell signaling. The energy-minimized 3D conformations of l-Glu-Hist derived from its chemical structure resulted in stabilization for Fe²⁺ chelating complexes. l-Glu-Hist accelerated the decrease of ferrous iron in the ferrous sulfate solution in a concentration-dependent mode and showed the ferroxidase-like activity at concentrations less than 3 mM in the phenanthroline assay, whereas in the concentration range 3–20 mM l-Glu-Hist restricted the availability of Fe²⁺ to phenanthroline due to binding of ferrous ions in chelating complexes. l-Glu-Hist showed stimulatory effect on phosphatidylcholine liposomal peroxidation (LPO) catalyzed by the superoxide anion radical (O₂⁻)-generating system (Fe²⁺ + ascorbate) at low (less or about 1 mM) l-Glu-Hist concentrations and both revealed the inhibitory effect on LPO in this system of high (10 mM) l-Glu-Hist concentration. The stimulation of LPO by l-Glu-Hist was related to the ability of peptidomimetic in small (0.05 mM) concentrations to release O₂⁻ free radicals as determined by the superoxide dismutase-inhibitable cytochrome c reduction assay. O₂⁻ release by l-Glu-Hist might result from its ferroxidase-like activity, while inhibition of LPO by l-Glu-Hist was caused by its chelating activity to Fe²⁺ ions, prevention of free radical generation and lipid hydroperoxide-degrading ability of 5–20 mM l-Glu-Hist. l-Glu-Hist released O₂⁻ in concentrations which stimulated [³H]-thymidine incorporation into DNA and proliferation of mouse spleen lymphocytes and mononuclear cells from human blood. l-Glu-Hist modulates the ability of oxygen free radicals to act as signaling agents at low concentrations, influencing gene expression. The structural peptide-like analogues of l-Glu-Hist such as l-Glu-Trp, carcinine (β-alanylhistamine), but not l-Pro-Glu-Trp were active in stimulating thymidine incorporation and in inducing proliferation of mononuclear cells as compared to mitogen concanavalin A at doses 2.5–25.0 μg/ml. Our data provide evidence that l-Glu-Hist may act as a very fast, specific and sensitive trigger for lymphocyte proliferation and immunoregulation. The cited abilities and further obtained in vivo results make Immudilin^® ((INCI: glutamylamidoethyl imidazole, aqueous solution), l-Glu-Hist) a useful immunoregulatory agent.