Molecular cytogenetic analysis of breast cancer: a combined multicolor fluorescence in situ hybridization and G-banding study of uncultured tumor cells
- 作者：Ferti ; Angeliki D. ; Stamouli ; Maria J. ; Panani ; Anna D. ; Raptis ; Sotirios A. ; Young ; Bryan D.
- 刊名：Cancer Genetics and Cytogenetics
- 出版年：2004
- 期刊代码：43_01654608
- 类别：med
- 出版时间：February, 2004
- 卷：149
- 期：1
- 页码：28-37
- 文件大小：434 K
摘要
In six patients with breast cancer, uncultured tumor cells were investigated with G-banding and multicolor fluorescence in situ hybridization (M-FISH). A large number of numerical and structural aberrations could be analyzed. Among other structural abnormalities, reciprocal, hidden and complex translocations were found. Recurrent t(1;10) and t(6;16), not previously described, were identified, as well as t(15;22). The latter was also found in additional cases among our unpublished breast carcinomas. The significance of t(15;22) for breast cancer is discussed, taking into account also data drawn from the literature. Reciprocal translocations were a prominent feature in a pseudodiploid lobular carcinoma. Hidden translocations on 6p22
p24 were detected with M-FISH. Involvement of 6p22p24 was observed in five cases. The analysis of various other translocations and different structural abnormalities revealed the following common breakpoints (according to frequency of involvement): 1p34p36, 3p12p13, 4p13→q11, 14p11→q11, 1q42, 8p11, 8q24, 10q22, 11q13, 11q23q24, 13q13, and 18p10p11. Loss of 3p and 1p34p36→pter and complete or partial loss of 13q and chromosome 17 were also found. With the combination of G-banding and M-FISH techniques, chromosome misclassification is avoided and the characterization of complex tumor karyotypes is more effective.
p24 were detected with M-FISH. Involvement of 6p22p24 was observed in five cases. The analysis of various other translocations and different structural abnormalities revealed the following common breakpoints (according to frequency of involvement): 1p34p36, 3p12p13, 4p13→q11, 14p11→q11, 1q42, 8p11, 8q24, 10q22, 11q13, 11q23q24, 13q13, and 18p10p11. Loss of 3p and 1p34p36→pter and complete or partial loss of 13q and chromosome 17 were also found. With the combination of G-banding and M-FISH techniques, chromosome misclassification is avoided and the characterization of complex tumor karyotypes is more effective.