Glycosyltransferase activity can be selectively modulated by chemical modifications of acceptor substrates
- 作者：Galan ; M. Carmen ; Dodson ; Christopher S. ; Venot ; Andre P. ; Boons ; Geert-Jan
- 关键词：Oligosaccharides ; Glycosyltransferase ; Chemical modifications ; Metabolic inhibition
- 刊名：Bioorganic and Medicinal Chemistry Letters
- 出版年：2004
- 期刊代码：10_0960894x
- 类别：ch
- 出版时间：May 3, 2004
- 卷：14
- 期：9
- 页码：2205-2208
- 文件大小：255 K
摘要
A range of N-acetyllactosamine derivatives, which are modified by a wide range of functionalities at C-2′ and C-6, have been synthesised and the kinetic parameters of transfer catalysed by recombinant α-2,6-sialyltransferase and α-1,3-fucoyltransferase VI determined. Several of the chemical modifications led to selective modulate the activity the enzymes and offer promising lead compounds for the development of oligosaccharide primers for selective metabolic inhibition of oligosaccharide biosynthesis.