X-linked inhibitor of apoptosis protein (XIAP) is a potent antagonist of
caspases, and functions as a ubiquitin-E3 ligase by itself and for
caspases. Recently, NEDD
8, a ubiquitin-like modifier, has been suggested to be used for modification of
caspase-7 mediated by XIAP. However, it is not clear whether
caspase-7 is a bona fide target for NEDD
8. Here we showed that no neddylation of
caspase-7 but that of XIAP itself was observed under the conditions in which
caspase-7 was modified with ubiquitin. These results reveal that XIAP does not function as a NEDD
8-E3 ligase for
caspase-7 in vivo.
Structured summary of protein interactions
with by ()
with by ()