The endocannabinoid and endovanilloid systems interact in the rat prelimbic medial prefrontal cortex to control anxiety-like behavior

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• 作者：Manoela V. Foga&ccedil ; a^a ; ^{manoelafogaca@usp.br} ; Daniele C. Aguiar^b ; Fabrí ; cio A. Moreira^b ; Francisco S. Guimarã ; es^a
• 关键词：Anandamide ; Anxiety ; Cannabinoid ; CB1 receptors ; Prefrontal cortex ; TRPV1 receptors ; Vanilloid
• 刊名：Neuropharmacology
• 出版年：2012
• 期刊代码：33_00283908
• 类别：pha
• 出版时间：August, 2012
• 卷：63
• 期：2
• 页码：202-210
• 文件大小：1125 K

摘要

Cannabinoid receptor 1 (CB₁) agonists usually induce dose-dependent biphasic effects on anxiety-related responses. Low doses induce anxiolytic-like effects, whereas high doses are ineffective or anxiogenic, probably due to activation of Transient Receptor Potential Vanilloid Type 1 (TRPV₁) channels. In this study we have investigated this hypothesis by verifying the effects of the CB₁/TRPV₁ agonist ACEA injected into the prelimbic medial prefrontal cortex (PL) and the participation of endocannabinoids in the anxiolytic-like responses induced by TRPV₁ antagonism, using the elevated plus-maze (EPM) and the Vogel conflict test (VCT). Moreover, we verified the expression of these receptors in the PL by double labeling immunofluorescence. ACEA induced anxiolytic-like effect in the intermediate dose, which was attenuated by previous injection of AM251, a CB₁ receptor antagonist. The higher and ineffective ACEA dose caused anxiogenic- and anxiolytic-like effects, when injected after AM251 or the TRPV₁ antagonist 6-iodonordihydrocapsaicin (6-I-CPS), respectively. Higher dose of 6-I-CPS induced anxiolytic-like effects both in the EPM and the VCT, which were prevented by previous administration of AM251. In addition, immunofluorescence showed that CB₁ and TRPV₁ receptors are closely located in the PL. These results indicate that the endocannabinoid and endovanilloid systems interact in the PL to control anxiety-like behavior.