Effects of a lectin-like protein isolated from Acacia farnesiana seeds on phytopathogenic bacterial strains and root-knot nematode

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• 作者：T. Santi-Gadelha<sup>asup><sup> ; sup><sup>santi.tatiane@gmail.comsup> ; B.A.M. Rocha<sup>bsup> ; C.A.A. Gadelha<sup>asup> ; H.C. Silva<sup>bsup> ; R.E.R. Castellon<sup>bsup> ; F.J.T. Gon&ccedil ; alves<sup>csup> ; D.O. Toyama<sup>dsup> ; M.H. Toyama<sup>esup> ; A.J.F. de Souza<sup>fsup> ; L.O.S. Beriam<sup>gsup> ; J.L. Martins<sup>hsup> ; P.P. Joazeiro<sup>isup> ; B.S. Cavada<sup>bsup><sup> ; sup><sup>bscavada@ufc.brsup>
• 关键词：Acacia farnesiana ; Lectin ; Xanthomonas ; Clavibacter ; Meloidogyne incognita
• 刊名：Pesticide Biochemistry and Physiology
• 出版年：2012
• 期刊代码：182_00483575
• 类别：bio
• 出版时间：May, 2012
• 卷：103
• 期：1
• 页码：15-22
• 文件大小：690 K

摘要

m>Acacia farnesianam> lectin-like protein (AFAL) showed bacterioestatic effects against m>Xanthomonas axonopodism> pv. m>passifloraem> (Gram-negative) and m>Clavibacter michiganensis michiganensism> (Gram-positive), with the latter being more sensitive. This effect is probably due to the ability of AFAL to interact with the bacterial cell wall where we observed that AFAL induced macroscopic change. The maximum bacterial growth inhibition was approximately 78%when incubated with Gram-negative strains, and as high as 92%percent for the Gram-positive one. The antibacterial effect of flavonoids (rutin, quercetin and morin) was also observed using low concentrations against both bacterial strains. Prior incubation of both with AFAL at high concentrations increases the inhibitory effect of flavonoids on bacterial growth. The potential use of AFAL as a control agent against the root-knot nematode m>Meloidogyne incognitam> was investigated as well, showing anti-nematode properties involving both egg hatching and motility. In the juvenile second-stage, AFAL showed reduction in larval mobility when measured against a control group. The results suggest that AFAL is effective against m>M. incognitam> and could be used as a component of integrated pest management programs. These data also suggest that lectins probably play a role in plant defense not only against invertebrate phytopathogens, herbivores and fungi but also against bacteria.