摘要
In an effort to study on the antibiotic resistance in bacteria, we first report the thermokinetic parameters of the imipenem hydrolysis with B1 subclasses metallo-尾-lactamase CcrA from Bacteroides fragilis. The values of activation free energy is 87.54 卤 0.03, 88.78 卤 0.03, 89.90 卤 0.04 and 91.06 卤 0.02 kJ mol鈭? at 293.15, 298.15, 303.15 and 308.15 K, respectively, the activation enthalpy is 19.10 卤 0.01 kJ mol鈭?, the activation entropy is 鈭?33.56 卤 0.09 J mol鈭? K鈭? and the apparent activation energy E is 21.598 kJ mol鈭?. The results reveal that the imipenem hydrolysis catalyzed by CcrA is an exothermic and spontaneous reaction with reaction order of 1.4, and has a lower and than the cefazolin hydrolysis with same enzyme.