Thermokinetic characterization of imipenem hydrolysis with metallo-尾-lactamase CcrA from Bacteroides fragilis

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• 作者：Le Zhai ; Ke-Wu Yang ; ^{kwyang@nwu.edu.cn} ; Cheng-Cheng Liu ; Hui-Zhou Gao ; Xia Yang ; Ying Shi ; Jing Wen
• 关键词：Microcalorimetry ; Metallo-尾-lactamase CcrA ; Antibiotic hydrolysis ; Imipenem ; Thermokinetic parameters
• 刊名：Thermochimica Acta
• 出版年：2012
• 期刊代码：110_00406031
• 类别：ch
• 出版时间：10 July, 2012
• 卷：539
• 期：Complete
• 页码：67-70
• 文件大小：190 K

摘要

In an effort to study on the antibiotic resistance in bacteria, we first report the thermokinetic parameters of the imipenem hydrolysis with B1 subclasses metallo-尾-lactamase CcrA from Bacteroides fragilis. The values of activation free energy is 87.54 卤 0.03, 88.78 卤 0.03, 89.90 卤 0.04 and 91.06 卤 0.02 kJ mol^鈭? at 293.15, 298.15, 303.15 and 308.15 K, respectively, the activation enthalpy is 19.10 卤 0.01 kJ mol^鈭?, the activation entropy is 鈭?33.56 卤 0.09 J mol^鈭? K^鈭? and the apparent activation energy E is 21.598 kJ mol^鈭?. The results reveal that the imipenem hydrolysis catalyzed by CcrA is an exothermic and spontaneous reaction with reaction order of 1.4, and has a lower and than the cefazolin hydrolysis with same enzyme.