Isolation of siRNA target by biotinylated siRNA reveals that human CCDC12 promotes early erythroid differentiation

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• 作者：Cuiqing Fan^a ; Lin Dong^a ; Ning Zhu^a ; ^b ; Yuan Xiong^a ; Jiewen Zhang^a ; Le Wang^a ; Yan Shen^a ; Xiaoyin Zhang^c ; Meihong Chen^a ; ^b ; ^{chenmhxc@gmail.com}
• 关键词：Erythroid differentiation ; CCDC12 ; Biotinylated siRNA ; K-562  ; cell
• 刊名：Leukemia Research
• 出版年：2012
• 期刊代码：186_01452126
• 类别：med
• 出版时间：June, 2012
• 卷：36
• 期：6
• 页码：779-783
• 文件大小：557 K

摘要

Erythroid differentiation is a tightly regulated multi-step process that has not been fully elucidated. We previously reported that a siRNA screened from random siRNA library, siRNA clone-67, induced erythroid differentiation in human erythroleukemia K-562 cell line. Here we identified that human CCDC12 (coiled-coil domain containing 12) is a target of siRNA clone-67, by target capture with biotinylated siRNA. Over-expression of CCDC12 in K-562 cell up-regulated the expression of CD235, 蓻-globin and 纬-globin, accelerated cell growth, and slightly down-regulated the expression of GATA-2. Knockdown of CCDC12 slowed down the cell growth. These data indicate that CCDC12 is a new participant that promotes early erythroid differentiation.