Methyl jasmonate reduces the survival of cervical cancer cells and downregulates HPV E6 and E7, and survivin

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• 作者：Elad Milrot ; Anna Jackman ; Tatiana Kniazhanski ; Pinhas Gonen ; Eliezer Flescher¹ ; Levana Sherman ; ^{lsherman@post.tau.ac.il}
• 关键词：Cervical cancer ; Apoptosis ; Survivin ; Mitochondrial superoxide ; HPV E6 and E7
• 刊名：Cancer Letters
• 出版年：2012
• 期刊代码：44_03043835
• 类别：med
• 出版时间：1 June, 2012
• 卷：319
• 期：1
• 页码：31-38
• 文件大小：928 K

摘要

The present study further investigated the mode of action of methyl jasmonate (MJ) in different cervical cancer cell lines. We show that in addition to the short term cytotoxicity, MJ effectively reduced the survival of cervical cancer cells (clonogenicity assays). MJ induced apoptosis in all cervical cancer cells. In some cell lines, MJ caused elevation of the mitochondrial superoxide anion, notably, in HeLa and CaSki. Changes in the expression of p53 and bax were variable, yet, downregulation of survivin was common to all cervical cancer cells. MJ significantly reduced the levels of the human papillomavirus (HPV) E6 and E7 proteins without alteration of the mRNA levels. Moreover, ectopic expression of E6, E7 or both in cervical cancer cells that lack HPV (C33A), did not alter significantly their response to MJ. Our studies point to MJ as an effective anticancer agent against a variety of cervical cancer cells acting through shared and different pathways to induce cell death regardless of the presence of HPV.