The G₂/M checkpoint phosphatase cdc25C is located within centrosomes

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• 作者：Corinna Busch ; Olivia Barton ; Eberhard Morgenstern ; Claudia Gö ; tz ; Jü ; rgen Gü ; nther ; Andreas Noll ; Mathias Montenarh
• 关键词：Centrosomes ; cdc25C ; Phosphatase ; CENP-F ; Mitosis
• 刊名：The International Journal of Biochemistry and Cell Biology
• 出版年：2007
• 期刊代码：127_13572725
• 类别：med
• 出版时间：2007
• 卷：39
• 期：9
• 页码：1707-1713
• 文件大小：1011 K

摘要

cdc25C is a phosphatase which regulates the activity of the mitosis promoting factor cyclin B/cdk1 by dephosphorylation, thus triggering G₂/M transition. The activity and the sub-cellular localisation of cdc25C are regulated by phosphorylation. It is well accepted that cdc25C has to enter the nucleus to activate the cyclin B/cdk1 complex at G₂/M transition. Here, we will show that cdc25C is located in the cytoplasm at defined dense structures, which according to immunofluorescence analysis, electron microscopy as well as biochemical subfractionation, are proven to be the centrosomes. Since cyclin B and cdk1 are also located at the centrosomes, this subfraction of cdc25C might participate in the control of the onset of mitosis suggesting a further role for cdc25C at the centrosomes.