Neonatal repetitive maternal separation causes long-lasting alterations in various neurotrophic factor expression in the cerebral cortex of rats

详细信息	查看全文 | 推荐本文 |

• 作者：Kyoung-Youl Lee^a ; ^b ; Takanori Miki^b ; ^{mikit@med.kagawa-u.ac.jp} ; Toshifumi Yokoyama^c ; Masaaki Ueki^d ; Katsuhiko Warita^b ; Shingo Suzuki^b ; Ken-Ichi Ohta^b ; Zhi-Yu Wang^e ; Mostofa Jamal^f ; Tomiko Yakura^b ; Jun-Qian Liu^b ; Naohisa Hosomi^g ; Yoshiki Takeuchi^b
• 关键词：Brain ; Development ; Stress ; BDNF ; TrkB ; IGF-1 ; IGF-1R
• 刊名：Life Sciences
• 出版年：2012
• 期刊代码：62_00243205
• 类别：imm
• 出版时间：20 April, 2012
• 卷：90
• 期：15-16
• 页码：578-584
• 文件大小：276 K

摘要

Aims

This study was carried out to examine the effects of early postnatal maternal separation stress on the development of the cerebral cortex with respect to time-dependent fluctuations of neurotrophic factor ligand and receptor expression.

Main methods

Wistar rats were separated from their mothers for 3 h per day during postnatal days (PND) 10 to 15. The cerebral cortex was analyzed by real-time RT-PCR for the evaluation of the expression of mRNA for brain-derived neurotrophic factor (BDNF), TrkB, insulin-like growth factor-1 (IGF-1), and type 1 IGF receptor (IGF-1R) on PND16, 20, 30, and 60.

Key findings

The expression of these neurotrophic factor ligands and receptors in the cerebral cortex was enhanced on PND16 and PND20, and then it returned to baseline levels on PND30. By PND60, however, the expression levels were attenuated.

Significance

The important implication of this study is the persistent abnormal fluctuation of neurotrophic factor expression for a prolonged period, triggered even after the brain growth spurt. Given that neurotrophic factors play important roles in brain development, it can be speculated that the altered expression of these factors induced by maternal separation may interrupt normal brain development and ultimately lead to functional disruption. However, the possibility of such changes leading to various functional disruptions and the underlying mechanisms involved require further study.