Cerebral endothelial derived vascular endothelial growth factor promotes the migration but not the proliferation of oligodendrocyte precursor cells in vitro

详细信息	查看全文 | 推荐本文 |

• 作者：Kazuhide Hayakawa^a ; ^b ; Ji Hae Seo^a ; ^b ; ^c ; Loc-Duyen D. Pham^a ; ^b ; Nobukazu Miyamoto^a ; ^b ; Angel T. Som^a ; ^b ; Shuzhen Guo^a ; ^b ; Kyu-Won Kim^c ; ^d ; Eng H. Lo^a ; ^b ; Ken Arai^a ; ^b ; ^{karai@partners.org}
• 关键词：Oligodendrocyte precursor cell ; Vascular endothelial growth factor ; Migration ; Proliferation ; Cerebral endothelial cell ; Neurovascular unit ; Oligovascular niche ; White matter
• 刊名：Neuroscience Letters
• 出版年：2012
• 期刊代码：52_03043940
• 类别：neu
• 出版时间：28 March, 2012
• 卷：513
• 期：1
• 页码：42-46
• 文件大小：578 K

摘要

In gray matter, cerebral endothelium is known to provide trophic support for neighboring cells such as neurons. However, signaling from cerebral endothelium to white matter cells remains to be elucidated. Here, we show that vascular endothelial growth factor (VEGF-A) secreted from cerebral endothelial cells promotes the migration but not the proliferation of oligodendrocyte precursor cells (OPCs). Cultured OPCs were obtained from newborn rat cortex, and treatment with conditioned culture media of cerebral endothelial cells increased the OPC proliferation and migration. Importantly, co-treatment with anti-neutralizing antibody for Flk-1 (VEGF-receptor2) inhibited OPC movement but did not affect OPC propagation. Western blot and flow cytometry analyses confirmed that our cultured cerebral endothelial cells produced VEGF-A and our cultured OPCs expressed Flk-1. Taken together, our current data suggest that cerebral endothelium is supportive for oligodendrocyte lineage cells and VEGF-A may participate in the endothelium-OPC cell-cell signaling. This phenomenon may be important for white matter homeostasis.