Neuroprotective effects of VEGF administration after focal cerebral ischemia/reperfusion: Dose response and time window

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• 作者：Jiping Yang^a ; ^{jipingyang2004@yahoo.com.cn} ; Li Guo^b ; Ruichun Liu^b ; Huaijun Liu^a
• 关键词：VEGF ; vascular endothelial growth factor ; MCAO ; middle cerebral artery occlusion ; ERK ; extracellular signal-regulated kinase ; ER ; endoplasmic reticulum ; MCA ; middle cerebral artery ; MRI ; magnetic resonance imaging ; DWI ; diffusion weighted imaging ; ADC ; ap
• 刊名：Neurochemistry International
• 出版年：2012
• 期刊代码：120_01970186
• 类别：bio
• 出版时间：May, 2012
• 卷：60
• 期：6
• 页码：592-596
• 文件大小：301 K

摘要

Administration of vascular endothelial growth factor (VEGF) has been shown to increase cerebral blood flow and reduce neurological damage after experimental ischemic brain injury. The purpose of this study was to examine the optimal dose and time window for the neuroprotective effect of VEGF when administrated after focal ischemia/reperfusion injury in rabbits. Focal cerebral ischemia/reperfusion was induced by the middle cerebral artery occlusion (MCAO) method. In a dose response experiment, low (1.25 ng/渭L), middle (2.5 ng/渭L) and high (5.0 ng/渭L) doses of VEGF were administered 2 h after MCAO by the route of perifocal region. The VEGF at a dose of middle (2.5 ng/渭L) displayed excellent effects on neuroprotective efficacy for focal cerebral ischemia/reperfusion injury. In another experiment, 2.5 ng/渭L VEGF was administered at times varying from 2 to 8 h after MCAO. Infarct volume, water content and neurological deficits were significantly reduced when VEGF was given at 2 and 3 h after injury. The protective effect was less when the same dose was given at the later times. Thus, the present findings indicated that VEGF reduced ischemic neuronal danger with a therapeutic time window within the first 3 h of transient MCAO and may be useful in the treatment of acute ischemic stroke in humans.