Strongly amphiphilic photosensitizers are not substrates of the cancer stem cell marker ABCG2 and provides specific and efficient light-triggered drug delivery of an EGFR-targeted cytotoxic drug

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• 作者：På ; l Kristian Selbo^a ; ^b ; ^c ; ^{Selbo@rr-research.no} ; Anette Weyergang^a ; Marius Strø ; mbo Eng^a ; Monica Bostad^a ; Gunhild M. Mæ ; landsmo^c ; ^d ; Anders Hø ; gset^b ; ^c ; Kristian Berg^a
• 关键词：ABCG2 ; Photochemical internalization ; Drug delivery ; Cancer stem cells ; MDR ; EGFR
• 刊名：Journal of Controlled Release
• 出版年：2012
• 期刊代码：25_01683659
• 类别：pha
• 出版时间：30 April, 2012
• 卷：159
• 期：2
• 页码：197-203
• 文件大小：810 K

摘要

A wide range of anti-cancer drugs are substrates of the ATP-binding cassette transporter ABCG2/CD338/BCRP/MXR, which is thought to play an important role in multi-drug resistance (MDR) and protection of cancer stem cells (CSC) against chemotherapeutics and photodynamic therapy (PDT). Hence, it is of importance to develop drugs that are not substrates of ABCG2. The aim of this study was to elucidate if photosensitizers utilized for the endo-lysosomal release drug delivery method photochemical internalization (PCI) are substrates for ABCG2. The breast carcinoma cell line MA11, with a Hoechst 33342 side population of > 50%was used as an ABCG2high model. The photosensitizer Pheophorbide A (PhA) and Hoechst 33342 were used as positive control substrates of ABCG2. ABCG2-inhibition by fumitremorgin C (FTC) did neither induce an increased accumulation of three different PCI-photosensitizers (di-sulfonated meso-tetraphenylporphine (TPPS_2a), di-sulfonated meso-tetraphenylchlorin (TPCS_2a) and di-sulfonated aluminium phtalocyanine (AlPcS_2a)) nor enhanced the photosensitization (P = 0.65 for TPCS_2a-PDT) of these PCI-based photosensitizers in the MA11 cells. The same results were also obtained with TPPS_2a in the malignant glioma cell line U87 having a SP of ~ 0.1%. In contrast, both uptake and PDT-induced cytotoxicity was strongly enhanced for PhA when combined with FTC (P < 0.001)). Specific and efficient light-controlled killing of EGFR+/ABCG2 + MA11 cells was obtained by PCI of the targeting toxin EGF-saporin. The novel data obtained in this study demonstrates that strongly amphiphilic photosensitizers used for PCI-based drug delivery are not substrates of ABCG2. This important findings warrant further development of the PCI technology as a strategy for efficient and site-specific eradication of MDR cells and CSC.