Ras Association Domain Family 1A: A Promising Prognostic Marker in Recurrent Nonmuscle Invasive Bladder Cancer

详细信息	查看全文 | 推荐本文 |

• 作者：Ji Sang Kim<sup>1sup><sup> ; sup><sup>2sup> ; Yunbyung Chae<sup>3sup> ; Yun-Sok Ha<sup>1sup><sup> ; sup><sup>4sup> ; Isaac Yi Kim<sup>4sup> ; Seok Soo Byun<sup>5sup> ; Seok-Joong Yun<sup>1sup> ; Wun-Jae Kim<sup>1sup><sup> ; sup><sup>2sup><sup> ; sup><sup>wjkim@chungbuk.ac.krsup>
• 关键词：Bladder cancer ; Methylation ; Prognosis ; RASSF1A
• 刊名：Clinical Genitourinary Cancer
• 出版年：2012
• 期刊代码：pca93_15587673
• 类别：med
• 出版时间：June, 2012
• 卷：10
• 期：2
• 页码：114-120
• 文件大小：405 K

摘要

ss="h4">Introduction

Aberrant methylation of promoter CpG islands is an important inactivation mechanism of tumor suppressors and tumor-related genes. Ras association domain family 1A (RASSF1A) promoter hypermethylation was shown to be associated with bladder cancer (BC), but its prognostic value remains unclear. The aim of the present study was to investigate the value of RASSF1A methylation as a prognostic marker in BC.

ss="h4">Materials and Methods

Primary BC tissues were obtained from 301 patients and included 186 specimens of nonmuscle invasive bladder cancer (NMIBC) and 115 specimens of muscle invasive bladder cancers (MIBC). RASSF1A hypermethylation was assessed using methylation-specific polymerase chain reaction (MS-PCR). The association between RASSF1A hypermethylation and clinicopathologic features, and the prognostic significance of RASSF1A hypermethylation were evaluated by Kaplan-Meier and multivariate Cox regression analyses.

ss="h4">Results

RASSF1A promoter hypermethylation was detected in 33.6%of BCs and occurred more frequently in MIBC (46.1%) than in NMIBC (25.8%) (P < .001). In NMIBC, RASSF1A methylation was associated with advanced tumor stage (P = .026) and high grade (P < .001). Among patients with recurrent NMIBC, RASSF1A methylation was associated with shorter time to progression by Kaplan-Meier analysis (log-rank test; P = .004) and identified as an independent predictor of cancer progression by multivariate Cox regression analysis (hazard ratio [HR], 8.559; P = .014).

ss="h4">Conclusions

Our results suggest that methylated RASSF1A may be a potential prognostic marker in patients with recurrent NMIBC.