CNP-1 (ARRD-17), a Novel Substrate of Calcineurin, Is Critical for Modulation of Egg-Laying and Locomotion in Response to Food and Lysine Sensation in Caenorhabditis elegans

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• 作者：Changhoon Jee¹ ; ² ; ^&dagger ; ; Tae-Woo Choi³ ; ^&dagger ; ; Karunambigai Kalichamy³ ; Jong Zin Yee³ ; Hyun-Ok Song² ; ⁴ ; Yon Ju Ji² ; Jungsoo Lee² ; Jin Il Lee¹ ; Noelle D. L'Etoile¹ ; Joohong Ahnn³ ; ⁵ ; ^{joohong@hanyang.ac.kr} ; Sun-Kyung Lee³ ; ⁵ ; ^{sunkyungl@hanyang.ac.kr}
• 关键词：TXNIP ; thioredoxin-interacting protein ; GST ; glutathione S-transferase ; GFP ; green fluorescent protein ; IP ; immunoprecipitation
• 刊名：Journal of Molecular Biology
• 出版年：2012
• 期刊代码：102_00222836
• 类别：imm
• 出版时间：30 March, 2012
• 卷：417
• 期：3
• 页码：165-178
• 文件大小：1798 K

摘要

Calcineurin is a Ca²⁺/calmodulin-dependent protein phosphatase involved in calcium signaling pathways. In Caenorhabditis elegans, the loss of calcineurin activity causes pleiotropic defects including hyperadaptation of sensory neurons, hypersensation to thermal difference and hyper-egg-laying when worms are refed after starvation. In this study, we report on arrd-17 as calcineurin-interacting protein-1 (cnp-1), which is a novel molecular target of calcineurin. CNP-1 interacts with the catalytic domain of the C. elegans calcineurin A subunit, TAX-6, in a yeast two-hybrid assay and is dephosphorylated by TAX-6 in vitro. cnp-1 is expressed in ASK, ADL, ASH and ASJ sensory neurons as TAX-6. It acts downstream of tax-6 in regulation of locomotion and egg-laying after starvation, ASH sensory neuron adaptation and lysine chemotaxis, that is known to be mediated by ASK neurons. Altogether, our biochemical and genetic evidence indicates that CNP-1 is a direct target of calcineurin and required in stimulated egg-laying and locomotion after starvation, adaptation to hyperosmolarity and attraction to lysine, which is modulated by calcineurin. We suggest that the phosphorylation status of CNP-1 plays an important role in regulation of refed stimulating behaviors after starvation and attraction to amino acid, which provides valuable nutritious information.