In vitro inhibition of Sclerotinia sclerotiorum by mixtures of azoxystrobin, SHAM, and thiram

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• 作者：Yabing Duan¹ ; ^{yabing0324@hotmail.com} ; Shengming Liu¹ ; Changyan Ge¹ ; Xijie Feng ; Changjun Chen ; Mingguo Zhou ; ^{mgzhou@njau.edu.cn}
• 关键词：Sclerotinia sclerotiorum ; Respiration inhibition ; Oxygen consumption ; Mycelial growth ; Salicylhydroxamic acid (SHAM)
• 刊名：Pesticide Biochemistry and Physiology
• 出版年：2012
• 期刊代码：182_00483575
• 类别：bio
• 出版时间：June, 2012
• 卷：103
• 期：2
• 页码：101-107
• 文件大小：437 K

摘要

The necrotrophic fungal phytopathogen Sclerotinia sclerotiorum (Lib.) de Bary has a broad host range and frequently causes destructive diseases. The extensive use of common fungicides to control these diseases has selected for resistance in populations of S. sclerotiorum. In this study, 105 isolates of S. sclerotiorum from different geographical regions in Jiangsu Province of China were characterized for baseline sensitivity to azoxystrobin, and the average EC₅₀ value was 0.2932 渭g/mL for mycelial growth. Of the mixtures of the fungicides thiram and azoxystrobin that were tested using an in vitro mycelial growth assay, the 1:4 ratio provided the greatest inhibition of S. sclerotiorum. When tested against nine isolates, the 1:4 mixture resulted in a mean synergy ratio of 2.31, indicating synergistic inhibition. Mycelial respiration was inhibited for about 2 h by azoxystrobin alone but for 48 h by the mixture of thiram and azoxystrobin. Salicylhydroxamic acid (SHAM, a known inhibitor of alternative respiration) also increased the inhibition of mycelial growth and respiration caused by azoxystrobin. These results suggest the need for further study of effects of combinations of azoxystrobin with thiram or SHAM in planta to evaluate their potential for management of diseases caused by S. sclerotiorum.