Effect of cultivation parameters on the production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and poly(3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate) by Cupriavidus necator using waste glycerol

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• 作者：Joã ; o M.B.T. Cavalheiro^a ; ^b ; Rodrigo S. Raposo^a ; ^b ; M. Catarina M.D. de Almeida^b ; ^c ; M. Teresa Cesá ; rio^a ; ^b ; Chantal Sevrin^d ; Christian Grandfils^d ; M.M.R. da Fonseca^b ; ^{manuela.fonseca@ist.utl.pt}
• 关键词：Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) ; Poly(3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate) ; Waste glycerol
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：May, 2012
• 卷：111
• 期：Complete
• 页码：391-397
• 文件大小：1647 K

摘要

Short-chain polyhydroxyalkanoate co-polymers (poly(3-hydroxybutyrate-co-4-hydroxybutyrate)) (P(3HB-co-4HB)) and terpolymers (poly(3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate)) (P(3HB-4HB-3HV)) were produced using high-cell density fed-batch cultures of Cupriavidus necator DSM 545. C-source for growth and 3HB synthesis was waste glycerol (GRP) from a biodiesel plant. Incorporation of 4HB monomers was promoted by 纬-butyrolactone (GBL). Propionic acid (PA), a stimulator of 4HB accumulation, increased the 4HB molar ratio 2-fold, but also acted as 3HV precursor, yielding P(3HB-4HB-3HV). Dissolved oxygen (DOC) was a key parameter for%PHA accumulation and volumetric productivity (Prod_vol). 4HB molar ratio increased in the presence of PA and with extended accumulation time. By manipulating DOC and cultivation time, P(3HB-4HB) with between 11.4 and 21.5 molar%of 4HB were attained. Similarly, P(3HB-4HB-3HV) was obtained with 4HB molar%between 24.8%and 43.6%and 3HV%from 5.6%to 9.8%. Mw varied between 5.5 脳 10⁵ and 1.37 脳 10⁶ Da. PHA production from GRP helps reducing production costs with concomitant GRP valorization.