Inhibition of Japanese encephalitis virus entry into the cells by the envelope glycoprotein domain III (EDIII) and the loop3 peptide derived from EDIII
- 作者：Chen Li ; Li-ying Zhang ; Ming-xia Sun ; Peng-peng Li ; Li Huang ; Jian-chao Wei ; Yi-lin Yao ; Hassan Isahg ; Pu-yan Chen ; puyanchen@yahoo.com.cn ; Xiang Mao ; xmao@njau.edu.cn
- 关键词：Japanese encephalitis virus ; Envelope protein domain III ; Antiviral peptide ; Attachment
- 刊名：Antiviral Research
- 出版年：2012
- 期刊代码：7_01663542
- 类别：imm
- 出版时间：May, 2012
- 卷：94
- 期：2
- 页码：179-183
- 文件大小：592 K
摘要
Japanese encephalitis virus (JEV) infection is a major cause of acute viral encephalitis both in humans and animals. The domain III of virus envelope protein (EDIII) plays important roles in interacting with host cell receptors to facilitate virus entry. In this study, recombinant JEV EDIII was expressed and purified. The protein showed the ability to inhibit JEV infection in BHK-21 cells with 50%inhibition at a concentration of 25 渭g/ml. Based on NMR structure of JEV EDIII, we chose several loop peptides that were reported to be related to receptor binding to test their possible inhibitory activities on virus infection. Our in vitro experiments demonstrated that one of the loop peptides (loop3) can prevent JEV infection with 50%inhibition at concentration of 10 渭M by interfering in virus attachment to the cells. Our in vivo experiments on mice showed the loop3 was the most protective peptide when administered before virus challenge. Therefore, the loop3 peptide may be served as basis for the development of novel antiviral agents against Japanese encephalitis virus or other flaviviruses infection.