Cloning and characterization of chsD, a chitin synthase-like gene of Aspergillus fumigatus
- 作者：Mellado ; Emilia ; Specht ; Charles A. ; Robbins ; Phillips W. ; Holden ; David W.
- 关键词：Chitin synthase ; Chitin ; Aspergillus ; Mutation
- 刊名：FEMS Microbiology Letters
- 出版年：1996
- 期刊代码：34_03781097
- 类别：imm
- 出版时间：September 15, 1996
- 卷：143
- 期：1
- 页码：69-76
- 文件大小：574 K
摘要
A chitin synthase-like gene (chsD) was isolated from an Aspergillus fumigatus genomic DNA library. Comparisons with the predicted amino acid sequence from chsD reveals low but significant similarity to chitin synthases, to otherN -acetylglucosaminyltransferases (NodC from Rhizopus spp., HasA from Streptococcus spp. and DG42 from vertebrates. Achs D− mutant strain constructed by gene disruption has a 20%reduction in total mycelial chitin content; however, no differences between the wild-type strain and the chsD− strain were found with respect to morphology, chitin synthase activity or virulence in a neutropenic murine model of aspergillosis. The results show that the chsD product has an important but inessential role in the synthesis of chitin in A. fumigatus.