Effects of polyphenols derived from fruit of Crataegus pinnatifida on cell transformation, dermal edema and skin tumor formation by phorbol ester application

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• 作者：Erl-Shyh Kao ; Chau-Jong Wang ; Wea-Lung Lin ; Chia-Yih Chu ; Tsui-Hwa Tseng
• 关键词：Crataegus pinnatifida ; 12-O-Tetradecanoylphorbol-13-acetate ; Skin tumor ; Transformation ; Osteopontin
• 刊名：Food and Chemical Toxicology
• 出版年：2007
• 期刊代码：20_02786915
• 类别：pha
• 出版时间：October 2007
• 卷：45
• 期：10
• 页码：1795-1804
• 文件大小：710 K

摘要

The dried fruits of Crataegus pinnatifida have been used traditionally as oriental medicine and local soft drink material recently. Previously, we demonstrated that C. pinnatifida exhibited anti-oxidation and anti-inflammatory potential. To clarify the active components in anti-transformation and anti-tumor promotion, we collected the polyphenol fraction (CF-TP) of hot-water extracts from dried fruits of C. pinnatifida for the following study. By anchorage-independent transformation assay, CF-TP significantly inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell transformation in JB6 P⁺ cells. Moreover, we found that CF-TP inhibited the expression of osteopontin (OPN), a transformational marker, and the activation of NF-κB and AP-1 induced by TPA in JB6 P⁺ cells. In addition, we evaluated the effect of CF-TP on TPA application to ICR mouse skin with measurement of H₂O₂ production, myeloperoxidase (MPO) activity, edema formation, epidermal thickness and leukocyte infiltration. As a result, CF-TP significantly inhibited the generation of reactive oxygen species (ROS) and the phenomena of inflammation induced by TPA. It also suppressed the expression of COX-2 and iNOS, and the activation of ornithine decarboxylase (ODC). Furthermore, CF-TP inhibited benzo[a]pyrene (B[a]P)/TPA-induced skin tumor formation and decreased the incidence of tumor. These results indicate that CF-TP possesses potential as a cancer chemopreventive agent against tumor promotion.