Bt-maize effects on biological parameters of the non-target aphid Sitobion avenae (Homoptera: Aphididae) and Cry1Ab toxin detection

详细信息	查看全文 | 推荐本文 |

• 作者：Ricardo Ramirez-Romero ; Nicolas Desneux ; Josette Chaufaux ; Laure Kaiser
• 关键词：Sitobion avenae ; ELISA ; Bt-maize ; Cry1Ab ; Non-target insect ; Risk assessment
• 刊名：Pesticide Biochemistry and Physiology
• 出版年：2008
• 期刊代码：182_00483575
• 类别：bio
• 出版时间：June 2008
• 卷：91
• 期：2
• 页码：110-115
• 文件大小：172 K

摘要

Bt-maize crop is increasingly used worldwide and the study of ecological side effects is a major subject in this domain. Under laboratory conditions, we determined Bt-maize effects on the non-target aphid Sitobion avenae (Fabricius) (Homoptera: Aphididae). We found no significant differences between S. avenae on MON810 and the near-isogenic line when alate offspring production, apterous survivorship, longevity, intrinsic rates of natural increase (r_m), finite rates of increase and doubling times were compared. No significant differences were found between treatments for apterous pre-reproductive and reproductive periods. Additionally, we used immunological tests (ELISA) to detect Cry1Ab protein in maize leaves and S. avenae nymphs. Results showed that Bt-maize leaves expressed 0.203 (±0.05) μg Cry1Ab/g leaf tissue (Mean ± SEM). No Cry1Ab protein was present in S. avenae nymphs developing on Bt or conventional maize. We conclude that Bt-maize does not affect the development of the non-target aphid S. avenae and that Cry1Ab toxin quantities in these aphids are nil, suggesting an inconsequential risk for natural enemies of this aphid species.