Double spiral detection channel for on-chip chemiluminescence detection

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• 作者：Khoi Seng Lok^a  ; [Author Vitae] ; Yien Chian Kwok^a ; ^{yienchian.kwok@nie.edu.sg}  ; [Author Vitae] ; Nam-Trung Nguyen^b ; ^{mntnguyen@ntu.edu.sg}  ; [Author Vitae]
• 关键词：Chemiluminescence ; Lab-on-chip ; Micro total analysis system ; Luminol ; Cobalt ; l-Cysteine ; Uric acid
• 刊名：Sensors and Actuators B ; Chemical
• 出版年：2012
• 期刊代码：161_09254005
• 类别：et
• 出版时间：5 July, 2012
• 卷：169
• 期：Complete
• 页码：144-150
• 文件大小：925 K

摘要

In this paper, we introduced a three-layered microchip that consists of a double spiral channel design for chemiluminescence (CL) detection and a passive micromixer to facilitate the mixing of reagents. The design with two overlapping spiral channels doubled the CL intensity emitted from the reaction as compared to the design with a single spiral channel. The addition of the passive micromixer improved the signal by 1.5 times. Luminol-based chemiluminescence reaction was used for the characterization of the device. This microchip was successfully tested with the determination of l-cysteine and uric acid.