Rapid determination of chloramphenicol in soft-shelled turtle tissues using on-line MSPD-HPLC-MS/MS

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• 作者：Yanbin Lu ; ^{luyanbin@zjgsu.edu.cn} ; Tinglu Zheng ; Xin He ; Xianjiang Lin ; Linyao Chen ; Zhiyuan Dai ; ^{dzy@zjgsu.edu.cn}
• 关键词：CAP ; chloramphenicol ; LC&ndash ; MS/MS ; liquid chromatography&ndash ; tandem mass spectrometry ; MSPD ; matrix solid-phase dispersion ; MRM ; multiple-reaction monitoring ; ESI ; electrospray ionization
• 刊名：Food Chemistry
• 出版年：2012
• 期刊代码：48_03088146
• 类别：abs
• 出版时间：1 September, 2012
• 卷：134
• 期：1
• 页码：533-539
• 文件大小：454 K

摘要

In this work, an analytical protocol based on on-line matrix solid-phase dispersion (MSPD) ultra-fast LC-MS/MS was successfully established, for rapid determination of chloramphenicol (CAP) present in soft-shelled turtle tissues. Trace level of CAP was analysed and separated on a core-shell C18 silica column in 2 min. The performance of the on-line MSPD-LC-MS/MS method was evaluated using multi-reaction monitoring (MRM) quantification mode. The calibration curve was linear over the range of 0.01-100 ng mL^鈭?. The mean values of relative standard deviation of intra- and inter-day ranging from 2.05%to 8.33%and from 3.05%to 10.17%were obtained, respectively. In the four fortified levels (1, 5, 7.5 and 10 ng), recoveries of CAP ranged from 92.05%to 98.07%with RSDs 猢?#xA0;4.20%. The proposed method has been applied successfully to the analysis of CAP in soft-shelled turtle tissue sample, which demonstrates that this new instrumental coupling is ultra-fast, sensitive, reliable and environmental friendly.