- 作者：Pei-Pei Li1 ; Dan-Dan Liu1 ; Yun-Jie Liu ; Shan-Shan Song ; Qing-Tong Wang ; Yan Chang ; Yu-Jing Wu ; Jing-Yu Chen ; Wen-Di Zhao ; Ling-Ling Zhang ; llzhang@ahmu.edu.cn ; Wei Wei ; wwei@ahmu.edu.cn
- 关键词：PI3K/Akt/mTOR ; Signaling ; BAFF/BAFF-R ; Collagen induced arthritis ; Paeoniflorin
- 刊名：Journal of Ethnopharmacology
- 出版年：2012
- 期刊代码：26_03788741
- 类别：pha
- 出版时间：7 May, 2012
- 卷：141
- 期：1
- 页码：290-300
- 文件大小：2767 K
Ethnopharmacological relevance
Paeoniflorin (Pae) is extracted from the root of paeonia lactiflora which have attracted attention for anti-rheumatic and immune modulating properties.Aim of the study
To investigate the role of PI3K/Akt/mTOR signaling mediated by BAFF/BAFF-R in antibodies production and the regulation of Pae on the signaling pathway in rats with collagen-induced arthritis (CIA).
Materials and methods
CIA rats were randomly separated into different groups and treated with Pae (25, 100 mg/kg) from day 18 to day 38 after immunization. The effects of Pae on B lymphocytes of CIA rats were evaluated by the levels of BAFF, anti-CII antibody, IgA, IgG and IgM, and the expressions of BAFF-R, PI3K, p-Akt and mTOR.
Results
In CIA rats, the levels of anti-CII antibody, IgA, IgG and IgM in serum enhanced, BAFF, BAFF-R, PI3K, p-Akt and mTOR were highly expressed. Pae (100 mg/kg) obviously decreased arthritis score, relieved ankle and paw swelling, improved spleen histopathology in CIA rats, decreased the levels of IgA, IgM, IgG and anti-CII antibody, and significantly decreased the expressions of BAFF, BAFF-R, PI3K, p-Akt and mTOR.
Conclusion
PI3K/Akt/mTOR signaling mediated by BAFF/BAFF-R participates in antibodies production by B lymphocytes of CIA rats. Pae had therapeutic effects on rats with CIA. These effects might be relative to regulating PI3K/Akt/mTOR signal mediated by BAFF/BAFF-R, and down regulate the antibodies production further.