Relevance of Exocytotic Glutamate Release from Retinal Glia

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• 作者：Michal Slezak¹ ; ² ; ¹⁶ ; Antje Grosche³ ; ¹⁶ ; Aurore Niemiec¹ ; ¹⁶ ; Naoyuki Tanimoto⁴ ; Thomas Pannicke³ ; Thomas  ; A. Mü ; nch⁵ ; ⁶ ; Britni Crocker⁵ ; ⁷ ; Philippe Isope¹ ; Wolfgang Hä ; rtig³ ; Susanne  ; C. Beck⁴ ; Gesine Huber⁴ ; Geraldine Ferracci⁸ ; ⁹ ; Martine Perraut¹ ; Michael Reber¹ ; Monique Miehe¹⁰ ; Valé ; rie Demais¹⁰ ; Christian Lé ; vê ; que⁸ ; ⁹ ; ¹¹ ; Daniel Metzger¹² ; Klaudia Szklarczyk² ; Ryszard Przewlocki² ; Mathias  ; W. Seeliger⁴ ; Dominique Sage-Ciocca¹³ ; Johannes Hirrlinger¹⁴ ; ¹⁵ ; Andreas Reichenbach³ ; Sophie Reibel¹³ ; Frank  ; W. Pfrieger¹ ; ^{fw-pfrieger@gmx.de}
• 刊名：Neuron
• 出版年：2012
• 期刊代码：45_08966273
• 类别：neu
• 出版时间：10 May, 2012
• 卷：74
• 期：3
• 页码：504-516
• 文件大小：2039 K

摘要

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Summary

Glial cells release molecules that influence brain聽development, function, and disease. Calcium-dependent exocytosis has been proposed as potential release mechanism in astroglia, but the physiological relevance of 鈥済liotransmission鈥?in聽vivo remains controversial. We focused on the impact of glial exocytosis on sensory transduction in the retina.聽To this end, we generated transgenic mice to block exocytosis by Cre recombinase-dependent expression of the clostridial botulinum neurotoxin serotype聽B light chain, which cleaves vesicle-associated membrane protein 1-3. Ubiquitous and neuronal toxin expression caused perinatal lethality and聽a reduction of synaptic transmission thus validating transgene function. Toxin expression in M眉ller cells inhibited vesicular glutamate release and impaired glial volume regulation but left retinal histology and visual processing unaffected. Our model to study gliotransmission in聽vivo reveals specific functions of exocytotic glutamate release in retinal glia.