Influence of myeloperoxidase-catalyzing reaction on the binding between myeloperoxidase and anti-myeloperoxidase antibodies

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• 作者：Peng-Cheng Xu ; Jian Hao ; Min Chen ; ^{leimeng@public3.bta.net.cn} ; Zhao Cui ; Ming-Hui Zhao
• 关键词：Antineutrophil cytoplasmic antibodies ; Myeloperoxidase ; Ceruloplasmin ; Catalysis
• 刊名：Human Immunology
• 出版年：2012
• 期刊代码：75_01988859
• 类别：bio
• 出版时间：April, 2012
• 卷：73
• 期：4
• 页码：364-369
• 文件大小：1350 K

摘要

In the current study, whether myeloperoxidase (MPO)-catalyzing reaction could influence the antigenicity of MPO was investigated. Hypochlorite acid, the main product of the catalytic reaction, could lower the binding between MPO-antineutrophil cytoplasmic antibodies (ANCA) and MPO when the available chlorine was higher than 0.031脳10^鈭? g/l. After MPO-catalyzing reaction with H₂O₂ lower than 0.469 g/l, the binding level between MPO-ANCAcontaining plasma and MPO increased slightly. The peak binding level was 1.135 卤 0.205 (expressed by the absorbance value at 405 nm). However, with the existence of hydrogen donor (o-phenylenediamine) in the reaction system, the peak binding level between MPO-ANCA-containing plasma and post-catalyzing MPO was significantly higher (1.367 卤 0.321 vs 1.135 卤 0.205, p = 0.023). Moreover, at the approximately physical concentration of H₂O₂ (0.02 g/l), MPO-ANCA exhibited higher titer to post-catalyzing MPO than to pre-catalyzing MPO (3.91 卤 0.84 vs 3.57 卤 0.84, p < 0.001, expressed as the lgT). These data demonstrated that MPO-catalyzing reaction could potentially increase the antigenicity of MPO.