Application of lignocellulolytic enzymes produced under solid state cultivation conditions
- 作者：Deepa Deswal ; Abha Sharma ; Rishi Gupta ; Ramesh Chander Kuhad ; kuhad85@gmail.com
- 关键词：Lignocellulolytic enzymes ; Saccharification ; ANF ; Chick feed ; Decolorization
- 刊名：Bioresource Technology
- 出版年：2012
- 期刊代码：11_09608524
- 类别：ce
- 出版时间：July, 2012
- 卷：115
- 期：Complete
- 页码：249-254
- 文件大小：364 K
摘要
In this paper, cellulose from brown-rot fungus Fomitopsis sp. RCK2010, thermostable and alkalostable xylanase from Bacillus pumilus MK001 and laccase from Ganoderma sp. rckk-02 were evaluated for (i) saccharification of alkali pretreated rice straw and wheat straw, (ii) upgradation of chick feed and (iii) decolorization of dyes, respectively. The cellulose from brown-rot fungus resulted in a sugar release of 151.48 and 214.11 mg/g, respectively, from rice straw and wheat straw, which was comparatively higher than the earlier reports. While xylan, one of the main anti-nutritional factors (ANFs) present in the chick feed was removed to an extent of 11.6 mg/g xylose sugars at 50 掳C using the thermostable xylanase. Besides, the treatment with thermostable xylanase also brought about a release of 0.85 (mg/g) of soluble phosphorous. Moreover, the laccase when used for the decolorization of Remazol Brilliant Blue R (RBBR) and xylidine ponceau cause almost complete decolorization in 2 and 4 h, respectively, depicting high rate of decolorization.