Electrochemical properties of carbon nanotubes–hydrogenase conjugates Langmuir–Blodgett films

详细信息	查看全文 | 推荐本文 |

• 作者：Ai-Rong Liu ; Tatsuki Wakayama ; Chikashi Nakamura ; Jun Miyake ; Nikolay A. Zorin ; Dong-Jin Qian
• 关键词：Hydrogenase ; Carbon nanotubes ; Langmuir– ; Blodgett films ; Electrochemistry ; Chronocoulometry
• 刊名：Electrochimica Acta
• 出版年：2007
• 期刊代码：30_00134686
• 类别：ch
• 出版时间：15 February 2007
• 卷：52
• 期：9
• 页码：3222-3228
• 文件大小：796 K

摘要

We report the preparation of Langmuir–Blodgett (LB) films composed of oxidized carbon nanotubes (CNTs) and hydrogenase (H₂ase) conjugates and their electrochemical properties. Both single-walled (SWNTs) and multi-walled CNTs (MWNTs) were used to form mixed monolayers with H₂ase on the Tris–HCl subphase surfaces. By using the LB method, the CNTs–H₂ase monolayers were transferred onto CaF₂ and indium tin oxide (ITO) electrode surfaces. The LB film modified electrodes showed a couple of waves centered at around −500 mV (versus Ag/AgCl), which corresponding to the redox reaction of [4Fe-4S]^2+/1+ clusters in the H₂ase. The current intensity was enhanced after co-assembly with CNTs. Because of the different diameters of CNTs, this current intensity was proportional to the scan rate (υ) for the electrodes modified with the LB films of pure H₂ase and SWNTs–H₂ase, but to the root of scan rate (υ^1/2) for those modified with the MWNTs–H₂ase LB film. The products of diffusion coefficient and concentration (D^1/2C) increased in the order of pure H₂ase, SWNTs–H₂ase, and MWNTs–H₂ase LB films.