Analytical and semipreparative high performance liquid chromatography enantioseparation of new substituted 1-thiocarbamoyl-3,5-diaryl-4,5-dihydro-(1H)-pyrazoles on polysaccharide-based chiral stationary phases in normal-phase, polar organic and reversed-p
- 作者：R. Cirilli ; A. Simonelli ; R. Ferretti ; A. Bolasco ; P. Chimenti ; D. Secci ; E. Maccioni and F. La Torre
- 关键词：Enantioselective HPLC ; Polysaccharide-based chiral stationary phases ; Chiralpak IA ; Semipreparative scale enantioseparation
- 刊名：Journal of Chromatography A
- 出版年：2006
- 期刊代码：47_00219673
- 类别：ch
- 出版时间：6 January 2006
- 卷：1101
- 期：1-2
- 页码：198-203
- 文件大小：217 K
摘要
The direct HPLC enantioseparation of five pairs of new chiral pyrazole derivatives on coated cellulose- and amylose-based chiral stationary phases (Chiralpak AD, Chiralcel OJ and Chiralcel OJ-RH) and new immobilised amylose-based Chiralpak IA CSP was performed. Very high enantioselectivity factor (α) values were achieved in polar organic and reversed-phase conditions by using OJ-RH as CSP. Chiralpak IA exhibited an excellent chiral resolving ability in normal-phase mode and it allowed the enantioseparation of analytes investigated with resolution factors (Rs) >20. Due to its bonded nature, it was successfully employed at analytical and semipreparative scale in combination with normal-phase eluents containing “non-standards” solvents such as acetone.