Evaluation of the OSCAR鈩?system for the production of monoclonal antibodies by CHO-K1 cells

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• 作者：Ana Rita Costa^a ; Maria Elisa Rodrigues^a ; Mariana Henriques^a ; ^{mcrh@deb.uminho.pt} ; David Melton^b ; Philip Cunnah^c ; Rosá ; rio Oliveira^a ; Joana Azeredo^a
• 关键词：Chinese hamster ovary cells ; Transfection ; OSCAR expression system ; Monoclonal antibody
• 刊名：International Journal of Pharmaceutics
• 出版年：2012
• 期刊代码：24_03785173
• 类别：pha
• 出版时间：1 July, 2012
• 卷：430
• 期：1-2
• 页码：42-46
• 文件大小：376 K

摘要

Biopharmaceutical production of complex recombinant protein therapeutics currently relies on mammalian cells. The development of high-yielding stable cell lines requires processes of transfection, selection and adaptation. With several technologies available, selection has been most frequently based on dihydrofolate reductase or glutamine synthetase systems, which can be very time-consuming. Due to the pressure to reduce development costs and speed up time to market, new technologies are emerging, as the promising OSCAR鈩?expression system that could provide more rapid development of high-yielding stable cell lines than the traditional systems. However, further evaluation of its application in a wider range of cell types and media is still necessary. In this study, application of OSCAR鈩?for the transfection of a CHO-K1 cell line with a monoclonal antibody was evaluated. OSCAR鈩?was reasonably fast and simple, without negative impact on cell growth characteristics. However, minigene selection was critical, with only pDWM128 working for the cell line assessed. Initial relatively high levels of production decreased significantly in the first few weeks of passing, remaining relatively stable although with low yield thereafter. The results suggest that more work is required to develop methodologies and prove that OSCAR鈩?has significant value to the bioproduction industry.