Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells

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• 作者：Yihua Cao^a ; ¹ ; Shuichi Kimura^b ; ¹ ; Takayuki Itoi^a ; Kohsuke Honda^a ; Hisao Ohtake^a ; Takeshi Omasa^a ; ^b ; ^{omasa@bio.tokushima-u.ac.jp}
• 关键词：Bacterial artificial chromosome (BAC) library ; Fluorescence in situ hybridization (FISH) ; Chinese hamster ovary (CHO) cells ; Genome ; Chromosome rearrangements
• 刊名：Methods
• 出版年：2012
• 期刊代码：103_10462023
• 类别：imm
• 出版时间：March, 2012
• 卷：56
• 期：3
• 页码：418-423
• 文件大小：709 K

摘要

Chromosome identification using Chinese hamster ovary (CHO) genomic bacterial artificial chromosome (BAC) clones has the potential to contribute to the analysis and understanding of chromosomal instability of CHO cell lines and to improve our understanding of chromosome organization during the establishment of recombinant CHO cells. Fluorescence in situ hybridization imaging using BAC clones as probes (BAC-FISH) can provide valuable information for the identification of chromosomes. In this study, we identified chromosomes and analyzed the chromosome rearrangement in CHO cells using BAC-FISH methods.