Isolation and identification of petromyzestrosterol, a polyhydroxysteroid from sexually mature male sea lamprey (Petromyzon marinus L.)

详细信息	查看全文 | 推荐本文 |

• 作者：Ke Li^a ; Michael J. Siefkes^b ; Cory O. Brant^a ; Weiming Li^a ; ^{liweim@msu.edu}
• 关键词：Cyclostoma ; Agnatha ; Polyhydroxylated estrogen ; Pheromone ; Electro-olfactogram recording
• 刊名：Steroids
• 出版年：2012
• 期刊代码：142_0039128x
• 类别：bio
• 出版时间：June, 2012
• 卷：77
• 期：7
• 页码：806-810
• 文件大小：335 K

摘要

Petromyzestrosterol (1), a novel polyhydroxylated steroid, was identified from water conditioned with sexually mature male sea lamprey (Petromyzon marinus), a jawless vertebrate animal. Along with this novel steroid, two known steroids, 7伪,12伪,24-trihydroxy-5伪-cholan-3-one-24-sulfate (3k PZS) and 7伪,12伪,24-trihydroxy-5伪-cholan-24-sulfate (PZS), were isolated. Structures of these compounds were unequivocally established by spectroscopic analyses and by comparison with spectra of known compounds. Electro-olfactogram recordings (EOG) showed that 1 at nanomolar concentrations was stimulatory for the olfactory epithelium of adult females. 3k PZS, known to function as a male sex pheromone, was more stimulatory than 1 for the female olfactory epithelia. The concentration-response curve of 3k PZS was exponential in shape with steep slopes between 10^-10 and 10^-6 mol L^-1. The concentration-response curve for 1 was shallower than that for 3k PZS.