Optimization of cultural conditions for growth associated chromate reduction by Arthrobacter sp. SUK 1201 isolated from chromite mine overburden

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• 作者：Satarupa Dey¹ ; ^{dey1919@gmail.com} ; A.K. Paul ; ^{amalk_paul@yahoo.co.in} ; ^{akpbot@caluniv.ac.in}
• 关键词：Arthrobacter ; Chromite mine overburden ; Hexavalent chromium ; Chromate reduction ; Chromium bioremediation
• 刊名：Journal of Hazardous Materials
• 出版年：2012
• 期刊代码：50_03043894
• 类别：ce
• 出版时间：30 April, 2012
• 卷：213-214
• 期：Complete
• 页码：200-206
• 文件大小：749 K

摘要

Arthrobacter sp. SUK 1201, a chromium resistant and reducing bacterium having 99%sequence homology of 16S rDNA with Arthrobacter sp. GZK-1 was isolated from chromite mine overburden dumps of Orissa, India. The objective of the present study was to optimize the cultural conditions for chromate reduction by Arthrobacter sp. SUK 1201. The strain showed 67%reduction of 2 mM chromate in 7 days and was associated with the formation of green insoluble precipitate, which showed characteristic peak of chromium in to energy dispersive X-ray analysis. However, Fourier transform infrared spectra have failed to detect any complexation of end products of Cr(VI) reduction with the cell mass. Reduction of chromate increased with increased cell density and was maximum at 10¹⁰ cells/ml, but the reduction potential decreased with increase in Cr(VI) concentration. Chromate reducing efficiency was promoted when glycerol and glucose was used as electron donors. Optimum pH and temperature of Cr(VI) reduction was 7.0 and 35 掳C respectively. The reduction process was inhibited by several metal ions and metabolic inhibitors but not by Cu(II) and DNP. These findings suggest that Arthrobacter sp. SUK 1201 has great promise for use in Cr(VI) detoxification under a wide range of environmental conditions.